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| Zhang,Fanfan; Ye,Yu; Song,Deping; Guo,Nannan; Peng,Qi; Li,Anqi; Zhou,Xingrong; Chen,Yanjun; Zhang,Min; Huang,Dongyan; Tang,Yuxin. |
| Abstract Background Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading to significant economic losses in the pork industry. Therefore, a rapid and sensitive method for detection of PDCoV in clinical samples is urgently needed. Results In this study, we developed a single-tube one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay specific for nucleocapsid gene to diagnose and monitor PDCoV infections. The detection limit of RT-LAMP assay was 1 × 101 copies of PDCoV, which was approximately 100-fold more sensitive than gel-based one-step reverse transcription polymerase chain... |
| Tipo: Journal article |
Palavras-chave: Porcine Deltacoronavirus (PDCoV); RT-LAMP; Rapid diagnosis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100222 |
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| Ribeiro,Tarsila C.R.; Kondo,Mario; Amaral,Ana Cristina C.; Parise,Edson Roberto; Bragagnolo Júnior,Maurício A.; Souza,Aécio Flávio Meirelles de. |
| In order to evaluate the accuracy of a urine reagent dipstick (Multistix 10SG®) to determine ascitic fluid leukocyte count, we prospectively studied 106 cirrhotic patients from April 2003 to December 2004, in two different centers (Federal University of São Paulo - UNIFESP-EPM and Federal University of Juiz de Fora - HU-UFJF) for the rapid bedside diagnosis of spontaneous bacterial peritonitis. The mean age 54 ± 12 years, there was a predominance of males (eighty-two patients, 77%), and alcohol was the most frequent etiology (43%). Forty-four percent of patients were classified as Child B and fifty-one as Child C (51%). Abdominal paracentesis was performed both in outpatient and inpatient settings and the Multistix 10SG® was tested. Eleven cases of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ascitic fluid; Rapid diagnosis; Dipstick; Bacterial peritonitis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100017 |
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| Silva,Pedro Eduardo Almeida da; Wiesel,Fernanda; Boffo,Maria Marta Santos; von Groll,Andréa; Mattos,Ivo Gomes de; Mejia,Glória; Robledo,Jaime. |
| Tuberculosis remains one of the major causes of mortality worldwide. The early detection of new cases is an important goal in the program of tuberculosis control. Several methodologies for rapid and accurate laboratorial diagnosis have been developed, however, some of these techniques are expensive or cumbersome, making their implementation in low-income regions unfeasible. In this study, the thin layer culture method was compared with conventional culture method and it was observed that it provides earlier results and a presumptive species identification, being adequate alternative method for rapid laboratory diagnosis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Rapid diagnosis; Mycobacterium tuberculosis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300007 |
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